外源糖对苹果果实山梨 毕业论文醇代谢相关酶活性的影响

外源糖对苹果果实山梨 毕业论文醇代谢相关酶活性的影响

摘要:以新红星苹果为试材,回收 果实圆片孵育体系研究了 外源糖对SDH和SOX 活性的影响。功效表白:在整个发育进程中,苹果 果实的SDH 活性与葡萄糖、果糖和蔗糖含量间不存在显著的相关性;而SOX活性与葡萄糖和果糖含量间存在极显著的相关性。 外源山梨醇和蔗糖均可提高发育前期和发育后期苹果果实的SDH和SOX活性,个中以山梨醇的诱导浸染最为显著。外源葡萄糖对SDH活性的增加具有显著的促进浸染, 硕士论文 ,但抑制SOX活性,而外源果糖对苹果果实SDH和SOX活性的诱导结果与葡萄糖刚好相反。功效还表白,外源糖对发育前期苹果果实SDH和SOX的诱导浸染均显著高于对发育后期苹果果实的诱导浸染。由此揣度,苹果果实中山梨醇代谢相关酶的活性变革受到可溶性糖的调控。
  要害词:苹果果实;外源糖;山梨醇脱氢酶;山梨醇氧化酶
   
  Effects of Soluble Sugars on Related Enzyme Activities of Sorbitol Metabolism in Developing Apple Fruit
  YE Cheng-rong, LIU Geng-sen, WANG Yong-zhang, LI Pei-huan, LIU Cheng-lian ,YUAN Yong-bing
  (College of Gardens and Horticulture, Qingdao Agriculture University, Qingdao, Shandong 266109, China)
  Abstract: The regulating effects of soluble sugars on activities of SDH and SOX were studied in ‘Starkrimson’ apple fruits through disk incubation. The results showed that the activities of SDH in apple fruit had no significant correlation with the concentrations of glucose, fructose and sucrose during the whole developmental period. However, the activities of SOX had significant positive correlation with glucose and fructose concentrations. The results also indicated that exogenous sorbitol and sucrose highly increased the activities of SDH and SOX, moreover, the exogenous glucose indicated the highest promoting effects on SDH activity, but inhibited the activity of SOX significantly. On the other hand, the exogenous fructose showed reverse regulating action on SDH and SOX activities compared with exogenous glucose. The exogenous study also indicated that the regulating effects of exogenous sugars on the fruit at early developmental stage were much higher compared with the fruit at late developmental stage. It is concluded that the metabolism of sorbitol in apple fruit was regulated by soluble sugars.
  Key words: apple fruit; exogenous sugar; sorbitol dehydrogenase; sorbitol oxidase
  
  果实所积聚糖的种类、含量及比率是抉择其品质及商品代价的重要因素[1]。近几年浩瀚研究功效表白, 毕业论文,可溶性糖不只作为代谢基质为植物发育提供能量和中间产品,并且还具有信号成果,可通过多条途径在差异程度上调理糖代谢相关酶的基因表达和酶活性变革[2-3]。山梨醇作为苹果光条约化产品的主要输出和运输形式[1, 4],在苹果果实内可被迅速转化[4]。山梨醇脱氢酶(SDH)和山梨醇氧化酶(SOX)是参加山梨醇代谢的要害酶,个中SDH将山梨醇还原成就糖,SOX将山梨醇氧化成葡萄糖[4-6]。发育进程中,苹果果实SDH和SOX活性变革与可溶性糖含量间的干系尚不清楚,本研究以新红星苹果为试材比拟举办了探讨与阐明。
  
  1 质料和要领
  
  1.1 质料
  试验于2009─2010年在青岛农业大学果树试验中心举办。以12年生新红星(Malus domestica Borkh. cv. Starkrimson)为试材,树势中庸,发展发育正常,通例田间打点。从花后隔断15 d取样本文来自(),未经答允,不得转载。1次,每次随机取50个果实,果肉用液氮速冻后置-80 ℃超低温冰箱贮存备用。别离在花后50 d和100 d举办外源糖孵育试验。
  1.2要领
  1.2.1可溶性糖的测定葡萄糖、果糖和蔗糖的测定回收高效液相色谱法(HPLC)[7]。柱温40 ℃,流速2 mL/min,RID示差检测器;活动相为乙腈∶水=83∶17,每次进样体积为20 μl,按照样品峰面积和尺度曲线计较蔗糖、葡萄糖和果糖含量。
  1.2.2果实圆片试验参照Beruter等[8]的要明确有窜改。将花后50 d和100 d的果实支解成直径1.0 cm,厚0.1 cm的圆片,在均衡缓冲液中均衡30 min后举办外源糖孵育试验。取5 g果肉圆片与20 mL别离插手浓度为100 mmol·L-1的葡萄糖、果糖、山梨醇、蔗糖的MES缓冲液殽杂(用100 mmol·L-1甘露醇调理各回响液的渗透势),放入50 mL的三角瓶中,在摇床上振荡回响16 h(25 ℃),测定SDH和SOX的活性。3次反复。
  1.2.3酶液的制备及酶活性测定称取1 g果肉于研钵内,加少量石英砂和5 mL的50 mmol·L-1 HEPES-NaOH(pH 7.5),冰浴研磨匀浆,12 000 g,4 ℃离心20 min。上清液用稀释10倍的提取缓冲液透析15 h。参照Yamaki等[5]的要领测定SDH和SOX的活性。
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